P-211: The Effects of Vasectomy on Histological Structure of Epididymis and Testis in Rat Model

Background: Vasectomy is a simple and highly effective contraceptive method with a low morbidity rate and an extremely low mortality rate. during the past decade millions of men have undergone vasectomy, but there have been surprisingly few studies of morphological effects of genital organs after vasectomy. The most important issues about vasectomy are degenerative changes in testicular tissue, epididymitis, and the occurrence of infectious mass. Also the reversibility of pregnancy should be mentioned. Then the aim of the present study was to evaluate effect of vasectomy on the testicular functions of rat. Materials and Methods: In this study 20 adult male wistar rats (220 ± 20 g) were randomly divided into two groups with one group was sham operated model and vasectomized group . All rats were sacrificed by CO2 inhalation 120 days after operation and the testis was removed and fixed in 10% formalin fluid and processed for paraffin embedding. Five μm thick sections were stained with HandE.Light microscopical analysis was made to evaluate the structural changes induced in the testis (p<0.05). Results: The histological and histomorphometrical study of testis confirmed that there was an obvious structural changes such as apearance of vacuolated edema in the interstitial connective tissue between the seminiferous tubules with increased hypertrophicf mass of leidig cells and decrease in seminiferous tubules diameter (STD), Tubule Differentiation Index (TDI) and spermatogenesis in the semniferous tubules (p<0.05). Conclusion: In this investigate we observed degenerative changes of testes tissue by pressure of accumulated sperms and semen fluid in epididymis on epithelial tissue of seminifrous tubules.